Table 1.
Primers used to generate overlapping cDNA fragments from SHFV-LVR RNA.
Primer | Sequence (5′ to 3′) |
---|---|
Fragment 1 | |
F-SP6-1 | act agt ATT TAG GTG ACA CTA TAG ATT AAA ATA AAA GTG TGA AGCa, b |
R-2343 | CAA CCA CCC AAT GGT CAG AGT CAA GG |
Fragment 2 | |
F-2307 | TAC GTT GTG CGC CTT GAC TCT GAC |
R-7991 | GCT TGC CAG ACA GAA ATT TGA GAC TG |
Fragment 3 | |
F-7858 | TGG TCT CTC CTC AGG TGA TC |
R-12306 | AGT CAT GTT GCC TGT AAT TGT CTC |
Fragment 4 | |
F-12190 | TAT GTC TAT CGT CCA CCA CTT GTC |
R-12983 | AGG TAT TTA GAA AGT CCA GTC ACG |
Fragment 5 | |
F-12662 | TCT GCT GGT TGG TAA AAT GCT CTC ACT CAC |
R-15717 | gcg gcc gcc gat cgt (T)42 AAT TAT GGT ATAb |
SP6 promoter consensus sequence is indicated by underlining.
Recognition sites for restriction enzymes SpeI in R2343 and NotI and PvuI in R-15717 are in lowercase.