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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mol Microbiol. 2014 Jul 10;93(3):426–438. doi: 10.1111/mmi.12671

Fig. 5. Spx mutants (F113A, and L114A) are degraded faster compared to Spx WT in the absence of YjbH in vivo.

Fig. 5

A. Western blot analysis of Spx level using anti-Spx antiserum in cells bearing the Physpank- spx (wt, F113A, or P114A) in yjbH spx null mutant background. Cells were grown to exponential phase, induced with 1 mM IPTG for 1 hr, and followed by chloramphenicol treatment. Cell samples were collected at the time points indicated, lysed, and analyzed by western blotting using anti-Spx serum.

B. Spx mutant (F113A) is not degraded in the absence of YjbH and ClpX in vivo. Western blot analysis of Spx level using anti-Spx antiserum in cells bearing the Physpank- spx (F113A) in yjbH spx clpX null mutant background. Cells were grown to exponential phase, induced with 1 mM IPTG for 1 hr, and followed by chloramphenicol treatment. Cell samples were collected at the time points indicated, lysed, and analyzed by western blotting using anti-Spx antiserum.