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. 2014 Jul 25;171(16):3814–3826. doi: 10.1111/bph.12738

Figure 1.

Figure 1

Detection of trypsin-like activity in the inflamed paw. Formalin (2.5%) (A,B), bradykinin (BK, 0.5 or 5 μg) (A,C), PAR2-AP (10 μg or 50 μg) (A,D) or NaCl (0.9%) were injected into mouse paws. After 1 h, paws were collected, homogenized and extracts (30 μg protein) were assayed for trypsin-like activity using the fluorogenic substrate BZiPAR. (A) Time course of substrate cleavage, indicating that all proinflammatory and algesic agents increased trypsin-like activity. (B–D) Effects of inhibitors on enzymatic activity normalized to control (inhibitor vehicle). Melagatran (MGT, 1 μM) but not SBTI (1 μM) abolished activity. n ≥ 3 experiments, *P < 0.05, significantly different from saline-treated tissues, #P < 0.05, significantly different from inhibitor vehicle.