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Purification and activity of mouse trypsin 4. (A) Purification and activation of mouse trypsinogen 4. Lane 1: Mouse trypsinogen 4 purified by immobilized metal ion chromatography. Lane 2: Enterokinase-activated mouse trypsin 4. Lane 3: Purified mouse trypsin 4 with degradation products. Products were identified by mass spectrometry. (B,C) The effects of graded concentrations of mouse trypsin 4 on [Ca2+]i measured in KNRK-mPAR2 (B) or KNRK-VC (C) cells. n = 3 experiments.
