Table 1.
Activity of tissue extracts for cleavage of PAR-derived peptides
| PAR-derived substrate | Intraplantar injection | Activity (%) | SD | Fold change |
|---|---|---|---|---|
| PAR2 | NaCl | 100.0 | 44.4 | 2.6 (P = 0.0251 vs. NaCl) |
| PAR2-AP | 264.0 | 68.2 | ||
| PAR4 | NaCl | 46.0 | 48.4 | 3.9 (P = 0.0231 vs NaCl) |
| PAR2-AP | 179.4 | 43.1 | ||
| PAR1 | NaCl | 57.3 | 6.4 | 1.7 (P = 0.0851 vs. NaCl) |
| PAR2-AP | 94.8 | 27.8 |
Extracts of paw prepared 60 min after intraplantar injection of PAR2-AP (50 μg) or NaCl (0.9%) were incubated with fluorogenic peptides derived from the canonical cleavage and activation sites for PAR2, PAR4 and PAR1. Activities were normalized against LDH activity of the extracts. The activity of NaCl-treated extracts for cleavage of PAR2-derived peptide was arbitrarily set to 100%. The change in activity between NaCl- (control) and PAR2-AP-treated animals, expressed in fold difference, is shown in the last column. Results are the mean ± SD of three independent experiments.