Figure 2.

Expression of NPY Y₁ receptors is increased in IPAH patients. (A) Representative gel image of PCR products for NPY ligand and receptors, in human lung tissue. PBGD serves as a positive control. Marker (M), lung homogenate (L), PA, PASMC and negative control (–). mRNA expression levels in PA from donor and IPAH lungs of (B) Y₁ receptors (C) NPY and (D) Y₅ receptors (n = 4–10). (E) Y₁ receptor mRNA expression level in donor and IPAH hPASMC (n = 9–10). (F) Correlation of Y₁ receptor gene expression in PA samples versus mean PAP (mPAP) in IPAH patient cohort. (G) Determination of circulating NPY levels in serum from donors and IPAH patients (n = 10). *P < 0.05.