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Inhibition of O-1918 induced increases of ERK1/2 phosphorylation in HEK293/GPR18 cells in the presence of the PKC inhibitor, Wortmannin (100 nm) and the PI3K inhibitor, BI (10 nM). Significance (**P < 0.01, ***0.001) was determined by one-way anova with Bonferroni correction.
