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. 2014 Jul 25;171(16):3908–3917. doi: 10.1111/bph.12746

Figure 7.

Figure 7

Immunocytochemistry of HEK293/GPR18 cells using anti-HA, and anti-GPR18 antibodies. Antibody staining is depicted in the left panel and nuclear staining using DAPI overlaid with the antibody staining image is depicted in the right panel. (A) Localization of receptor on the plasma membrane of live, non-permeabilized HEK293/GPR18 cells using anti-HA antibodies. (B) Intracellular localization of GPR18 receptors using anti-HA antibodies in live permeabilized cells. (C) Fixed, permeabilized cells depicting intracellular GPR18 receptors using anti-GPR18 antibodies. (D) Specificity of anti-HA and anti-GPR18 antibodies is demonstrated by a lack of staining in fixed, permeabilized wild-type HEK293 cells.