Table 2.
Potency (pEC50) and maximal response (Emax) values for peptide agonists of the glucagon receptor in yeast assays
| Ligand | GPA1/Gαs | GPA1/Gαi | ||
|---|---|---|---|---|
| pEC50a | Emaxb | pEC50a | Emaxb | |
| Glucagon | 8.0 ± 0.1 | 101 ± 6.1 | 8.0 ± 0.3 | 98 ± 8.1 |
| Oxyntomodulin | 7.8 ± 0.2 | 85 ± 10.4 | 7.6 ± 0.2 | 85 ± 6.8 |
Values generated through fitting of a three-parameter logistic equation and represent the mean ± SEM from at least five independent experimental repeats.
a
The negative logarithm of the agonist concentration required to generate half the maximal response.
b
The maximal response to the ligand expressed as a percentage of that obtained in the absence of antagonist.