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Number of Positive Nuclei Detected by Double Immunofluorescence of Cultured Fibers
| Antibodies tested | Reactivity of nuclei | Day 2 |
Day 3 |
||
|---|---|---|---|---|---|
| No. | % | No. | % | ||
| Anti-MyoD/Anti-PCNA | MyoD+ only | 36 | 9.39 | — | — |
| PCNA+ only | 18 | 4.70 | — | — | |
| MyoD+/PCNA+ | 329 | 85.90 | — | — | |
| Anti-MyoD/Anti-myogenin | MyoD+ only | — | — | 11 | 2.69 |
| Myogenin+ only | — | — | 344 | 84.31 | |
| MyoD+/myogenin+ | — | — | 53 | 12.99 | |
| Total positive nuclei | 383 | 408 | |||
| Total fibers | 60 | 60 | |||
