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. Author manuscript; available in PMC: 2014 Aug 11.
Published in final edited form as: Nat Methods. 2012 Sep 16;9(10):981–984. doi: 10.1038/nmeth.2175

Figure 3. HSQC spectra of recycled ACP-15N in various acyl states overlayed with apo-ACP-15N.

Figure 3

All spectra were collected on the same protein sample. (a) 15N-1H -HSQC of the originally prepared apo-ACP-15N (black) is overlayed with the HSQC of octanoyl-ACP-15N (blue). (b) HSQC of regenerated apo-ACP-15N (red) overlayed with the original apo-ACP-15N preparation (black). (c) HSQC of butanoyl-13C4-ACP-15N (orange) is overlayed with the original apo-ACP-15N (black). (d) HSQC of octanoyl-8-13C1-ACP-15N (lavender) is overlayed with the original apo-ACP-15N (black). Full spectra are available in Supplementary Information (Supplemtary Figs. 9-14).