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. 2014 Jul 21;111(31):11479–11484. doi: 10.1073/pnas.1407034111

Fig. 2.

Fig. 2.

(A and B) H2 oxidation and evolution by oxygen-limited cultures of M. smegmatis mc2155 and derivatives. H2 mixing ratios were measured on entry into stationary phase due to oxygen limitation and at regular intervals over 40 h. (A) WT and triple mutant compared with single hydrogenase KO strains. (B) DosR mutant compared with double hydrogenase KO mutants. (C and D) H2 recycling by cultures of M. smegmatis mc2155 and derivatives. All cultures were limited for O2 over 5 d. They were subsequently spiked with (C) 0.5% O2 or (D) 1 mM sodium fumarate (degassed). H2 mixing ratios were measured on spiking of culture and at regular intervals over 40 h. The initial headspace mixing ratios were adjusted to 55 ppmv. (E and F) Measurement of NAD/NADH ratios in oxygen-limited cultures of M. smegmatis (E) during midexponential phase before oxygen depletion and (F) 5 d following entry into stationary phase due to oxygen limitation. *P < 0.05, **P < 0.01 difference relative to WT bars. In all panels, error bars show SD from three biological replicates.