Fig. 4.

Off-target effects of Cas9/LTR-A/B on human genome. (A) SURVEYOR assay shows no indel mutations in predicted/potential off-target regions in human TZM-bI and U1 cells. LTR-A on-target region (A) was used as a positive control and empty U6-CAG vector (U6) as a negative control. (B−D) Whole-genome sequencing of LTR-A/B stable TZM-bI subclone showing the numbers of called indels in the U6-CAG control and LTR-A/B samples (B), detailed information on 10 called indels near gRNA target sites in both samples (C), and distribution of off-target called indels (D).