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Subcellular localizations of subclade A and B NST proteins. NST coding sequences were fused at their C terminus to the YFP and transiently expressed in tobacco leaves. All six candidates localized to moving dot-like structures typical for the Golgi apparatus and colocalized with the cis-Golgi marker α-Man consistent with a role in nucleotide sugar transport across the Golgi membrane. (Scale bars: 25 μm.)
