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. 2014 Jul 22;111(31):11563–11568. doi: 10.1073/pnas.1406073111

Fig. 4.

Fig. 4.

Time- and concentration-dependent nucleotide sugar uptake into proteoliposomes. Time courses of UDP-Rha (A) and UDP-Gal (B) uptake. Proteoliposomes, preloaded with UMP, were incubated with the respective nucleotide sugar at a concentration of 50 μM for the indicated time points at 25 °C. Kinetics of UDP-Rha (C) and UDP-Gal (D) transport. Proteoliposomes, preloaded with UMP, were incubated with their respective nucleotide sugar at varying concentrations (0.5–400 μM) for 3 min at 25 °C. Values were normalized to the actual NST protein content present in proteoliposome preparations and represent the mean and SD of two independent experiments. pYES-DEST52, yeast transformed with the empty vector.