Fig. 2.
Experimental set-up for in vivo identification of the Arabidopsis sulfenome. (A) Cell cultures overproducing the YAP1C/YAP1A probes treated with H2O2 as described (see main text). (B and C) Proteins isolated and subjected to a two-step purification procedure based on IgG-protein G and SBP affinity. Numbers indicate the sequence of elution steps. (D) LC-MS/MS analysis of proteins after elution. Comparison of interactors between the negative control probes of YAP1C and YAP1A potentially undergoing cysteine sulfenic acid (-SOH) formation under oxidative stress.