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. 2014 Jul 21;111(31):11491–11496. doi: 10.1073/pnas.1406693111

Fig. 5.

Fig. 5.

Insertion of IS6110 upstream phoPR in M. bovis B strain suppresses the phoPR-bovis deficiency. (A) Schematic representation of the genetic structure at the phoPR locus in the M. bovis B strain relative to “classic” M. bovis and M. tuberculosis strains. The positions of SNPs specific to the animal-adapted and M. africanum L6 and of the IS6110 insertion site are indicated in brackets. Red lines indicate individual SNPs identified between pairwise-compared genomes. Blue lines indicate the boundaries of IS6110. (B) qRT-PCR analysis of phoPR expression in M. tuberculosis H37Rv strain, the ΔphoPR mutant, and the recombinant M. tuberculosis strain expressing the phoPR-B allele. (C) Western blot analysis of PhoP from WT and phoPR-B expressing strains. (D) qRT-PCR analysis of expression of PhoP regulon genes in the indicated strains. (E) TLC analysis of lipids extracted from [14C] propionic acid-labeled cultures. (F) Survival of SCID mice infected with ∼4 × 103 cfu of the indicated strains (five mice per group). Curves are significantly different between the phoPR-bovis expressing strain and the phoPR-TB– and phoPR-B–expressing strains (P < 0.005, according to the log-rank (Mantel– Cox) test).