Fig. 1.

Distribution of fluorescent auxin analogs in Arabidopsis root. (A) Structures of the auxins NAA and IAA and their fluorescent analogs NBD-NAA and NBD-IAA. (B and C) Distribution of NBD-auxins in Arabidopsis roots. Six-day-old wild-type seedlings were treated with medium containing NBD-auxins for 15 min. Six-day-old DR5::GUS (B, a and b) and DR5::GFP seedlings (C) were incubated with auxins for 4 h or 6 h, respectively. Six-day-old DII-VENUS seedlings were treated with 10 μM auxinole for 6 h and then incubated with NAA for 10 min. The pictures in C are confocal images. The values presented in parentheses indicate the concentration of chemicals (μM). (Scale bar, 100 μm.)