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. 2014 Jul 17;111(31):11371–11376. doi: 10.1073/pnas.1410983111

Fig. 2.

Fig. 2.

Effects of CLP or FLAP knockdown on 5LO product formation in the absence of exogenous AA. Control and stable knockdown cells were differentiated as described. (A) Differentiated cells (∼2 × 106 cells in 1 mL of PGC buffer) were incubated with 5 µM ionophore A23187 for 10 min at 37 °C. (B) Differentiated cells (∼2 × 106 cells in 1 mL of PGC buffer) were first primed with PMA 100 nM at 37 °C for 10 min and then stimulated with 5 µM ionophore (A23187) for another 10 min. (C) Differentiated cells (∼4 × 106 cells in 1 mL of PGC buffer) were first primed with LPS (1 µg/mL) for 20 min and then stimulated with fMLP (1 µM) for another 10 min. Data are mean ± SD of two independent experiments; n = 6 (A and B) or n = 5 (C). ***P < 0.001; **P < 0.005.