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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Clin Periodontol. 2014 Jun 6;41(8):733–747. doi: 10.1111/jcpe.12262

Table 2. Relative quantitative proteome analysis by mass spectrometry of human GCF from healthy (controls) and periodontal disease subjects using stable-isotope-labeling chemistries, mTRAQ and ICAT.

(A); Relative quantitative proteome analysis of human GCF from healthy versus disease subjects led to the identification and quantification of 180 proteins. The proteins are grouped according to their functional categories with relative quantitative values reflected as ratio (perio/healthy) = mean ± confidence interval. The values of confidence interval (Cl) were calculated as described under methods using the standard deviation (STD), sample number and a 95% level of confidence. S = also found in serum proteome (Schenk et al., 2008). (B); Identification of proteins only in healthy or periodontal GCF derived samples during quantitative proteome analysis using stable-isotope-labeling chemistries. 58 proteins were identified without relative quantification, of which 26 and 32 distinct proteins were found only in GCF of healthy or periodontal samples, respectively.

Table 2A. Relative quantitative proteome analysis of GCF from healthy and periodontal disease subjects using stable-isotope-labeling chemistries. S.D. = standard deviation, # runs = number of times a given protein was identified, # peptides = number of distinct peptides identified in each run for a given protein and used for quantification, SNS = statistically not significant.
Accession # GCF Proteins mTRAQMean ±S.D P value ICATMean ±S.D. P value # Runs # Peptides
Apoptosis and Signal Transduction
SP02647 Apolipoprotein A-I 1.25 ± 0.20 <0.03 9 4
SP04114 Apolipoprotein B-100 1.44 ± 0.46 SNS 5 3
P53355 Death-associated protein kinase 2.5 ± 1.14 SNS 4 2
Q9BTC0 Death-inducer obliterator 1 3.75 ± 2.40 SNS 3 2
P98164 Low-density lipoprotein receptor-related protein 2 0.89 ± 0.19 SNS 3 2
Q8IZF6 Probable G-protein coupled receptor 112 0.95 ± 0.48 SNS 3 2
Q13972 Ras-specific guanine nucleotide-releasing factor 1 0.82 ± 0.58 SNS 3 2
P21817 Ryanodine receptor 1 0.63 ± 0.34 SNS 4 2
Q92736 Ryanodine receptor 3 1.09 ± 0.97 SNS 3 2
O60292 Signal-induced proliferation-associated 1-like protein 3 0.55 ± 0.52 SNS 3 2
Q6ZSZ6 Teashirt homolog 1 0.97 ± 0.83 SNS 4 2
P62736 Actin, aortic smooth muscle 1.39 ± 0.32 SNS 10 15
SP60709 Actin, cytoplasmic 1 1.62 ± 0.72 <0.01 7 4
Q562M3 Actin-like protein 1.69 ± 0.45 <0.03 5 2
O94833 Bullous pemphigoid antigen 1 1.13 ± 0.93 SNS 3 3
SP06396 Gelsolin 1.25 ± 0.18 SNS 3 2
SP02533 Keratin, type I cytoskeletal 14 1.04 ± 0.30 SNS 13 13
SP08779 Keratin, type I cytoskeletal 16 0.88 ± 0.11 SNS 4 2
P04264 Keratin, type II cytoskeletal 1 1.20 ± 0.32 SNS 8 2
Q01546 Keratin, type II cytoskeletal 2 oral 0.95 ± 0.20 SNS 4 2
SP35908 Keratin, type II cytoskeletal 2 epidermal 1.47 ± 0.15 <0.001 17 3
P12035 Keratin, type II cytoskeletal 3 1.30 ± 0.35 SNS 7 4
P19013 Keratin, type II cytoskeletal 4 1.01 ± 0.31 SNS 9 6
SP13647 Keratin, type II cytoskeletal 5 1.09 ± 0.11 <0.02 8 6
SP02538 Keratin, type II cytoskeletal 6A 1.11 ± 0.15 SNS 16 10
P08729 Keratin, type II cytoskeletal 7 1.31 ± 0.03 <0.05 4 2
SP13646 Keratin, type I cytoskeletal 13 1.42 ± 0.27 <0.001 16 15
P20929 Nebulin 0.77 ± 0.35 SNS 4 3
Q8WXH0 Nesprin-2 2.04 ± 0.86 <0.03 6 2
Extracellular Structural Proteins
O95996 Adenomatous polyposis coli protein 2 0.60 ± 0.18 SNS 3 2
SP39060 Collagen alpha-1(XVIII) chain 0.71 ± 0.16 SNS 3 2
Q9ULU4 Protein kinase C-binding protein 1 1.18 ± 0.39 SNS 3 3
P12107 Collagen alpha-1(XI) chain 1.75 ± 0.72 SNS 6 2
Q05707 Collagen alpha-1(XIV) chain 0.43 ± 0.07 <0.02 3 2
P02462 Collagen alpha-1(IV) chain 0.87 ± 0.41 SNS 7 2
P12107 Collagen alpha-1(XI) chain 1.75 ± .96 SNS 6 2
Q05707 Collagen alpha-1(XIV) chain 0.43 ± 0.07 <0.004 3 2
Q8NFW1 Collagen alpha-1(XXII) chain 1.46 ± 0.75 SNS 3 4
Q8IZC6 Collagen alpha-1(XXVII) chain 0.72 ± 0.36 SNS 5 2
P02462 Collagen alpha-4(IV) chain 0.9 ± 0.21 SNS 4 3
S08123 Collagen alpha-2(I) chain 0.91 ± 0.05 SNS 7 3
P29400 Collagen alpha-5(IV) chain 1.14 ± 0.42 SNS 8 10
SP00738 Haptoglobin 1.45 ± 0.08 <0.02 1.12 ± 0.22 SNS 5 3
P24158 Myeloblastin 1.17 ± 0.16 SNS 7 2
Q5SZK8 FRAS1-related extracellular matrix protein 2 0.34 ± 0.01 <0.01 3 3
P13645 Keratin, type I cytoskeletal 10 0.79 ± 0.38 SNS 6 2
Q8WXI7 Mucin-16 1.14 ± 0.59 SNS 5 7
Q9HC84 Mucin-5B 1.63 ± 0.83 SNS 1.26 ± 0.35 SNS 7 8
Q14517 Protocadherin Fat 1 1.38 ± 0.59 SNS 5 2
Q96MS0 Roundabout homolog 3 0.72 ± 0.43 SNS 5 2
Q96QU1 Photocadherin 15 1.35 ± 0.15 SNS 4 3
SP02768 Serum albumin 2.40 ± 0.67 <0.0001 2.21 ± 0.68 <0.01 19 41
Q86UP0 Cadherin-24 1.62 ± 0.12 <0.003 1.68 ± 0.23 <0.02 4 2
Hydrolytic Enzymes and Proteinases
Q9UDR5 Alpha-aminoadipic semialdehyde synthase, 1.62 ± 0.38 SNS 3 2
P16050 Arachidonate 15-lipoxygenase 0.94 ± 0.14 SNS 3 2
Q86UQ4 ATP-binding cassette sub-family A member 13 1.37 ± 0.28 SNS 7 4
O00408 cGMP-dependent 3′,5′-cyclic phosphodiesterase 1.17 ± 0.32 SNS 3 2
Q9Y5K2 Kallikrein-4 (enamel matrix serine proteinase 1) 2.16 ± 0.45 <0.002 9 3
SP14618 Pyruvate kinase isozymes M1/M2 3.15 ± 2.14 SNS 5 3
SP07477 Trypsin-1 1.37 ± 0.16 SNS 11 4
P35030 Trypsin-3 1.00 ± 0.53 SNS 1.18 ± 0.15 SNS 10 4
A4D1T9 Peptidase S1 domain-containing protein LOC136242 2.67 ± 0.73 <0.001 5 2
A2RTX5 Probable threonyl-tRNA synthetase 2, cytoplasmic 1.25 ± 0.59 SNS 3 2
O95714 Probable E3 ubiquitin-protein ligase HERC2 1.03 ± 0.70 SNS 3 3
Q5S007 Leucine-rich repeat serine/threonine-protein kinase 2 0.74 ± 0.21 SNS 4 2
Q6P4F7 Rho GTPase-activating protein 11A 0.99 ±1.02 SNS 3 2
Q8IVL0 Neuron navigator 3 1.17 ± 0.61 SNS 6 3
P36871 Phosphoglucomutase-1 0.69 ± 0.22 SNS 3 2
Q9P212 1-Phosphatidylinositol-phosphodiesterase ε-1 1.65 ± 0.31 <0.03 6 2
Q9BYE2 Transmembrane protease, serine 13 1.53 ± 0.35 SNS 8 2
P30048 Thioredoxin-dependent peroxide reductase 1.69 ± 0.25 <0.04 7 2
SP04406 Glyceraldehyde-3-phosphate dehydrogenase 2.35 ± 0.45 <0.02 7 3
P08246 Leukocyte elastase 1.71 ± 0.18 <0.002 4 2
Q9UIQ6 Leucyl-cystinyl aminopeptidase 0.94 ± 0.28 SNS 3 2
Inflammatory and Immune Response
P04083 Annexin A1 1.41 ± 0.17 <0.001 13 8
SP01024 Complement C3 1.44 ± 0.68 SNS 5 4
SP01857 Ig gamma-1 chain C region 1.85 ± 0.58 <0.0001 1.98 ±0.61 <0.003 19 11
SP01859 Ig gamma-2 chain C region 1.63 ± 0.30 SNS 1.64 ±0.38 <0.01 6 3
SP01861 Ig gamma-4 chain C region 2.34 ± 1.72 SNS 4 2
SP01834 Ig kappa chain C region 1.34 ± 0.43 SNS 2.19 ± 0.83 SNS 8 4
SP01842 Ig lambda chain C regions 1.57 ± 0.48 SNS 1.7 ± 0.37 <0.05 5 5
SP02788 Lactotransferrin 1.65 ± 0.24 <0.04 1.61 ± 0.33 <0.05 8 3
P01833 Polymeric immunoglobulin receptor 1.4 ± 0.23 SNS 3 3
P05164 Myeloperoxidase 2.12 ± 0.51 <0.01 1.13 ± 0.27 SNS 6 4
SP59665 Neutrophil defensin 1 1.67 ± 0.37 <0.03 1.26 ± 0.12 <0.04 7 3
SP05109 Protein S100-A8 2.34 ± 0.65 <0.0001 1.25 ± 0.30 SNS 13 13
SP06702 Protein S100-A9 2.41 ± 0.51 <0.0001 1.89 ± 0.26 <0.01 14 8
Intracellular Protein/Nucleotide Binding
Q9UPY3 Endoribonuclease Dicer 2.42 ± 0.55 SNS 4 2
Q8IVF2 AHNK2 HUMAN Protein AHNAK2 1.24 ± 0.33 SNS 3 3
Q6S8J3 ANKRD26-like family C member 1A 1.36 ± 0.32 <0.02 11 6
P13010 ATP-dependent DNA helicase 2 subunit 2 0.5 ± 0.29 SNS 3 2
Q9NVP1 ATP-dependent RNA helicase DDX18 2.1 ± 2.60 SNS 3 2
Q9NRL2 Bromodomain adjacent to zinc finger domainprotein 1A 1.03 ± 0.26 SNS 3 2
Q2M1Z3 Cdc42 GTPase-activating protein 2.39 ± 0.48 <0.004 7 2
Q7Z7A1 Centriolin 1.09 ± 0.36 SNS 5 3
Q8TD26 Chromodomain-helicase-DNA-binding protein 6 1.48 ± 0.04 <0.01 3 2
Q5H945 Chromosome 1 open reading frame 9 0.63 ± 0.12 SNS 3 2
Q14999 Cullin-7 (CUL-7) 3.05 ± 0.4 <0.01 3 2
P16333 Cytoplasmic protein NCK1 2.05 ± 2.44 SNS 3 2
Q9H1X3 DnaJ homolog subfamily C member 25 1.28 ± 0.85 SNS 3 2
Q92621 Nuclear pore complex protein Nup205 1.32 ± 0.09 SNS 9 2
P52948 Nuclear pore complex protein Nup98-Nup96 2.82 ± 1.84 SNS 3 2
Q12830 Nucleosome-remodeling factor subunit BPTF 2.92 ± 0.22 <0.001 3 2
Q8NG31 Protein CASC5 1.06 ± 0.45 SNS 4 2
Q8IVF4 Dynein heavy chain 10 1.29 ± 0.08 SNS 3 2
Q9UFH2 Dynein heavy chain 17, axonemal 0.80 ± 0.14 SNS 4 3
Q8TE73 Dynein heavy chain 5 0.98 ± 0.22 SNS 3 2
Q8WXX0 Dynein heavy chain 7, axonemal 0.95 ± 0.23 SNS 3 2
O60447 Ecotropic viral integration site 5 protein homolog 1.38 ± 1.85 SNS 3 2
SP13639 Elongation factor 2 0.63 ± 0.17 SNS 5 2
SP21333 Filamin-A 0.86 ± 0.15 SNS 7 2
Q3V6T2 Girdin 2.69 ± 0.12 <0.03 3 2
Q9BXL5 Hemogen 2.35 ± 0.09 <0.03 3 2
SP69905 Hemoglobin subunit alpha 1.42 ± 0.13 <0.01 7 4
SP68871 Hemoglobin subunit beta 1.29 ± 0.11 <0.01 8 6
P0C0S8 Histone H2A type 1 1.92 ± 0.53 <0.004 7 2
Q96A08 Histone H2B type 1-A 1.34 ± 0.55 SNS 7 2
P62807 Histone H2B type 1-C/E/F/G/I 1.18 ± 0.12 SNS 3 2
P68431 Histone H3.1 1.07 ± 0.15 SNS 6 4
Q09666 Neuroblast differentiation-associated protein AHNAK 1.01 ± 0.03 SNS 5 4
Q7Z2Y8 Interferon-induced very large GTPase 1 1.05 ± 0.11 SNS 7 3
Q9UKX3 Myosin-13 1.67 ± 0.14 <0.03 3 2
SP62805 Histone H4 1.58 ± 0.33 <0.01 9 4
Q149N8 E3 ubiquitin-protein ligase SHPRH 1.39 ± 0.27 SNS 5 2
Q5THR3 EF-hand calcium-binding domain-containing protein 6 0.51 ± 0.10 SNS 3 2
Q9BXW9 Fanconi anemia group D2 protein subunit BPTF 2.16 ± 0.18 <0.03 3 2
Q96ST3 Paired amphipathic helix protein 1.28 ± 0.18 SNS 3 2
Q9C0D5 Protein TANC1 1.45 ± 0.18 SNS 5 3
O00360 Putative p150 2.37 ± 0.36 <0.05 3 2
Q9C0B0 RING finger protein unkempt 3.67 ± 1.32 SNS 4 2
Q9NSC2 Sal-like protein 1 0.85 ±0.70 SNS 3 2
O75691 Small subunit processome component 20 homolog 1.03 ± 0.18 SNS 3 2
P53804 Tetratricopeptide repeat protein 3 1.49 ± 0.61 SNS 3 2
O95359 Transforming acidic coiled-coil-containing protein 2 0.96 ± 0.13 SNS 6 2
Q9HCJ0 Trinucleotide repeat-containing gene 6C protein 0.74 ± 0.31 SNS 4 3
O94782 Ubiquitin carboxyl-terminal hydrolase 1 1.35 ± 0.57 SNS 3 2
Q96RL7 Vacuolar protein sorting-associated protein 13A 2.03 ± 0.75 <0.01 5 3
Q9UNX4 WD repeat-containing protein 3 0.78 ± 0.22 SNS 4 2
Q5JSH3 WD repeat-containing protein 44 1.23 ± 0.48 SNS 3 2
Q15911 Zinc finger homeobox protein 3 0.49 ± 0.19 SNS 3 2
Q86UP3 Zinc finger homeobox protein 4 1.14 ± 0.92 SNS 3 3
Q86YH2 Zinc finger protein 280B 0.66 ± 0.45 SNS 3 2
O94822 Zinc finger protein 294 1.23 ± 0.53 SNS 3 3
Q5T7W0 Zinc finger protein 618 1.16 ± 0.12 SNS 4 3
Proteins Falling Into Other Categories
P40145 Adenylate cyclase type 8 1.11 ± 0.43 SNS 5 2
Q9ULX6 A-kinase anchor protein 8-like 1.57 ± 0.86 SNS 3 2
P46013 Antigen KI-67 1.03 ± 0.71 SNS 4 4
Q5W041 Armadillo repeat-containing protein 4.32 ± 0.36 <0.001 3 2
P31513 Dimethylaniline monooxygenase [N-oxide-forming] 3 0.26 ± 0.17 <0.03 5 3
Q8WY64 E3 ubiquitin-protein ligase MYLIP 1.62 ± 0.83 SNS 4 2
Q9BUH6 Uncharacterized protein C9orf142 1.67 ± 0.23 <0.03 3 2
SQ8WZ4 Titin 2.37 ± 0.93 <0.001 8 10
A0AVI2 Fer-1-like protein 5 1.04 ± 0.12 SNS 3 2
Q5CZC0 Fibrous sheath-interacting protein 2 2.16 ± 1.15 SNS 4 2
Q17R60 Interphotoreceptor matrix proteoglycan 1 0.97 ± 0.04 SNS 5 2
Q9P041 HSPC-109 1.12 ± 0.12 SNS 0.91 ± 0.21 SNS 7 3
Q8WVZ9 Kelch repeat and BTB domain-containing protein 7 0.82 ± 0.51 SNS 4 2
Q9H825 Methyltransferase-like protein 8 1.24 ± 0.12 SNS 5 2
Q9NU22 Midasin 0.67 ± 0.17 SNS 3 2
Q5VTT5 Myomesin-3 0.14 ± 0.04 <0.001 3 2
Q8IVL1 Neuron navigator 2 0.51 ± 0.31 SNS 3 2
Q9P2E3 NFX1-type zinc finger-containing protein 1 0.54 ± 0.65 SNS 4 2
A8MV47 Uncharacterized protein ENSP00000380627 0.93 ± 0.11 SNS 6 2
Q8NG94 Olfactory receptor 11H1 1.08 ± 0.26 SNS 3 2
SP13796 Plastin-2 1.37 ± 0.34 SNS 4 2
Q96KK3 Potassium voltage-gated channel subfamily S member 0.73 ± 0.22 SNS 3 2
SP07737 Profilin-1 0.92 ± 0.24 SNS 7 3
Q658L4 Putative uncharacterized protein DKFZp666E157 1.13 ± 0.17 SNS 3 2
Q5VUG0 Scm-like with four MBT domains protein 2 0.44 ± 0.15 <0.04 3 2
SP02787 Serotransferrin 1.07 ± 0.12 SNS 1.59 ± 0.33 SNS 9 9
Q6IQ55 Tau-tubulin kinase 2 0.95 ± 0.33 SNS 5 3
Q5T6L9 Transmembrane protein C6orf70 1.55 ± 0.65 SNS 3 2
Q6ZXV5 Transmembrane and TPR repeat-containing protein 3 8.68 ± 6.6 SNS 3 2
Q0VAA2 Uncharacterized protein C14orf166B 1.24 ± 0.06 SNS 3 2
Q15878 Voltage-dependent R-type Ca+2 channel subunit α-1E 1.28 ± 0.91 SNS 6 2
Q8N4N8 Kinesin-like protein KIF2B 2.69 ± 0.37 <0.001 2.88 ± 0.26 <0.001 7 5
Protease and enzyme inhibitors
SP01009 Alpha-1-antitrypsin 1.88 ± 0.84 <0.01 12 9
SP04080 Cystatin-B 1.42 ± 0.56 SNS 8 2
SP01023 Alpha-2-macroglobulin 1.51 ± 0.60 SNS 9 7
Table 2B. Proteins found only in GCF derived from healthy periodontal sites labeled by light stable-isotope or periodontal sites labeled by heavy stable-isotope.
Proteins found only in GCF of healthy sites
Accession # Protein name Accession # Protein name
P49641 Alpha-mannosidase IIx P26045 Tyrosine-protein phosphatase non-receptor type 3
Q9NR09 Baculoviral IAP repeat-containing protein 6 P08579 U2 small nuclear ribonucleoprotein B″
Q14CN2 Calcium-activated chloride channel regulator 4 Q6NSZ9 Zinc finger protein 498
Q14031 Collagen alpha-6(IV) Q8WXB4 Zinc finger protein 606
O15061 Desmuslin P33908 Mannosyl-oligosaccharide-1,2-α-mannosidase IA
Q5T4S7 E3 ubiquitin-protein ligase UBR4 Q8TCU4 Alstrom syndrome protein 1
P29322 Ephrin type-A receptor 8 Q9UQ05 Potassium voltage-gated channel subfamily H 4
Q86XX4 Extracellular matrix protein FRAS1 P20648 Potassium-transporting ATPase alpha chain 1
Q0JRZ9 FCH domain only protein 2 Q6ZRV2 Protein FAM83H
Q13233 Mitogen-activated protein kinase kinase kinase 1 Q5TBA9 Protein furry homolog
P41218 Myeloid cell nuclear differentiation antigen Q4ZG55 Protein GREB1
Q01804 OTU domain-containing protein 4 Q9NSE7 Putative ATP-binding cassette transporter sub-family C member 13
P56645 Period circadian protein homolog 3 P23471 Receptor-type tyrosine-protein phosphatase zeta
Proteins found only in GCF of periodontal sites
Q65ZQ1 Anti-colorectal carcinoma heavy chain A7KAX9 Rho GTPase-activating protein 32
P12956 ATP-dependent DNA helicase 2 subunit 1 Q96AG3 Solute carrier family 25 member 46
P98160 Basement membrane-specific heparan sulfate proteoglycan core protein Q9HCB6 Spondin-1
P55285 Cadherin-6 Q9H2G2 STE20-like serine/threonine-protein kinase
Q9P2D1 Chromodomain-helicase-DNA-binding protein 7 Q5T1R4 Transcription factor HIVEP3
P06681 Complement C2 Q6UXZ0 Transmembrane and immunoglobulin domain-containing protein 1
Q9NY74 Ewing’s tumor-associated antigen 1 Q5T3F8 Transmembrane protein 63B
Q9UKT4 F-box only protein 5 OS=Homo sapiens O75382 Tripartite motif-containing protein 3
Q9BUJ2 Heterogeneous nuclear ribonucleoprotein U-like protein 1 A6NGQ3 Uncharacterized protein OBSCN
P01877 Ig alpha-2 chain C region Q9Y487 V-type proton ATPase 116 kDa subunit a isoform 2
Q8NFY9 Kelch repeat/BTB domain-containing protein 8 Q9UBW7 Zinc finger MYM-type protein 2
A4D0S4 Laminin subunit beta-4 Q5VU65 Nuclear pore membrane glycoprotein 210-like
Q9NS15 Latent-transforming growth factor β-binding protein 3 Q9H7F0 Cation-transporting ATPase 13A3
O00562 Membrane-associated phosphatidylinositol transfer protein 1 Q9UPA5 Protein bassoon
Q7Z5P9 Mucin-19 P46940 Ras GTPase-activating-like protein IQGAP1
P21439 Multidrug resistance protein 3 Q15303 Receptor tyrosine-protein kinase erbB-4
Table 2C. Relative quantitative bacterial and yeast proteome analysis of GCF from healthy and periodontal disease subjects using stable-isotope-labeling chemistries. S.D. = standard deviation, # runs identified = number of times a given protein was identified, # peptides identified = number of distinct peptides identified for a given protein and used for quantification, SNS = statistically not significant.
Accession # Bacterial proteins mTRAQMean ±S.D. P value # Runs Identified # Peptides Identified
K0ZHN4 Uncharacterized protein (Streptococcus sp) 3.87 ± 1.2 <0.02 2 4
U2JW31 Helicase protein (Porphyromonas gingivalis) 2.36 ± 1.4 SNS 2 4
M9VKY7 Sec-independent translocase (Propionbacterium acnes) 4.33 ± 3.45 SNS 2 2
B7GQL2 Uncharacterized protein ( Bifidobacterium longum ) 1.80 ± 0.75 SNS 2 3
U2JW31 Oxidoreductase (Porphyromonas gingivalis) 2.86 ± 0.91 <0.04 2 3
G6FFC9 Chromosmal partition protein Smc (Lactococcus lactis) 14.11 ±9.22 SNS 2 4
U2IB04 RelA/SpoT family protein (Porphyromonas gingivalis) 3.30 ± 1.12 <0.02 3 4
F1YR10 Glycine dehydrogenase (decarboxylating) (Acetobacter pomorum) 2.38 ± 0.44 <0.03 2 2
U2JN88 Outer membrane protein (Porphyromonas gingivalis) 2.34 ± 0.66 <0.03 2 3
U2JKX9 Uncharacterized protein (Porphyromonas gingivalis) 5.33 ± 4.2 SNS 2 4
G6FCL3 DNA topoisomerase (Lactococcus lactis) 1.10 ± 0.67 SNS 2 4
N6X320 Glutamate-tRNA ligase (Actinomyces cardiffensis) 2.38 ± 0.11 <0.04 2 2
G6FBX5 DNA-directed DNA polymerase (Lactococcus lactis) 1.83 ± 1.66 SNS 2 4
G6FAW1 GMP synthase (glutamine-hydrolyzing) (Lactococcus lactis) 4.25 ± 5.36 SNS 2 3
M9VHN8 Uncharacterized protein (Propionibacterium acnes) 1.29 ± 1.33 SNS 2 2
B7GN98 Uncharacterized protein (Bifidobacterium longum) 5.63 ± 0.94 <0.04 3 2
G6FFE2 Putative uncharacterized protein (Lactococcus lactis) 2.28 ± 0.61 <0.03 2 4
F9E9H1 aminopeptidase (Streptococcus sanguinis) 14.36 ±12.25 SNS 2 2
U2K2Z6 GTP-binding protein typeA (Porphyromonas gingivalis) 2.66 ± 1.8 SNS 2 2
Q6MQE9 Serine proteinase (peptidase) (Bdellovibrio bacteriovorus) 4.50 ± 4.10 SNS 2 2
Q4YIL3 Leucine amidopeptidase PfLAP (Plasmodium chabaudi) 2.50 ± 0.85 <0.04 2 3
K1A3W6 PTS system, Lactose/cellobiose-specific IICB (Enterococcus sp) 1.50 ± 0.33 SNS 2 2
M9VQL8 Uncharacterized protein (Propionibacterium acnes) 0.87 ± 0.12 <0.02 2 2
D7W9K3 Glycosyl hydrolase (Corynebacterium genitalium) 3.50 ± 1.2 <0.05 2 2
D9ZEW0 Putative carbohydrate-active enzyme (uncultured) 2.42 ± 0.60 <0.05 2 3
L8A4U1 PTS system, sucrose subfamily IIABC (Enterococcus faecium) 1.18 ± 0.13 SNS 2 2
F1YWU8 Glutamate synthase large chain (Acetobacter pomorum) 1.37 ± 0.34 SNS 2 3
U2IF16 Putative glycerate kinase (Porphyromonas gingivalis) 0.95 ± 0.31 SNS 2 2
F1YUN4 Formamidase (Acetobacter pomorum) 5.64 ± 0.2 <0.01 2 2
F1YSW6 Glutamate-ammonia-ligase adenyltransferase (Acetobacter pomorum) 4.88 ±2.85 SNS 2 2
Q8KHM3 Bifunctional protein GlmU (Fusobacterium nucleatum) 1.78 ± 0.45 <0.02 2 5
P31224 Multidrug efflux pump subunit AcrB (Escherichia coli) 2.34 ± 2.12 SNS 2 2
P49331 Glucosyltransferase-S (Streptococcus mutans) 1.21 ± 0.51 SNS 2 4
B7GTQ1 ABC transporter permease component (Bifidobacterium longum) 3.61 ± 4.75 SNS 2 2
G6EUF8 Putative uncharacterized protein (Lactobacillus lactis bulgaricus) 0.53 ± 0.22 <0.03 2 2
K1A813 Glycosyl transferase family protein (Enterococcus sp) 5.7 ± 1.7 <0.01 2 4
K1AX94 Cell wall surface anchor family protein (Enterococcus sp) 3.78 ± 3.69 SNS 2 2
B7GUQ5 Phosphatase (Bifidobacterium longum-infants) 14.1 +9.4 SNS 2 2
K1A149 Surface associated protein (Streptococcus sp) 1.81 ± 1.30 SNS 2 3
K0ZUJ1 Cell wall surface anchor family protein (Streptococcus sp) 4.40 ± 3.20 SNS 2 3
M9VPN0 Cysteine synthase (Propionibacterium acnes) 1.10 ± 0.42 SNS 2 3
K1A3L8 Nicotinate phosphorybosyltransferase (Enterococcus sp) 1.43 ± 1.33 SNS 2 2
G6FCY9 Putative uncharacterized protein (Lactococcus lactis) 2 4
Yeast Proteins
V5N887 Beta-actin (Chiloscylium puntatum) 16.50 ± 9.80 SNS 2 3
E7Q8H9 Poly (A) polymerase Trf5p (Saccharomyces cerevisiae) 1.06 ± 0.17 SNS 4 3
E3UAT6 Actin (Cladosporium cladosporioides) 1.80 ± 0.82 SNS 3 7
P14741 Translation initiation factor eIF2B (Saccharomyces cerevisiae) 3.39 ± 1.04 <0.02 2 2
N1P505 DNA polymerase (Saccharomyces cerevisiae) 1.20 ± 0.74 SNS 2 3
Q06593 Oligopeptide transporter (Saccharomyces cerevisiae) 1.17 ± 0.75 SNS 3 4
P14235 Actin (Candida albicans) 6.7 ± 2.67 SNS 2 4
N1NWJ3 Predicted protein Tcb1p(Saccharomyces cerevisiae) 0.94 ± 0.55 SNS 2 3
Q6FNY7 DNA polymerase (Candida glabrata) 1.20 ± 0.74 SNS 2 3
T1WEZ8 Actin (Collectotrichum truncotum) 1.6 + 0.58 <0.003 2 4
C7BFY6 Histone H3 protein (Tristoma integrum) 1.34 + 0.19 <0.0001 3 4