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. Author manuscript; available in PMC: 2014 Aug 11.
Published in final edited form as: Dev Biol. 2012 Jan 8;363(1):266–278. doi: 10.1016/j.ydbio.2011.12.046

Figure 3. Tribolium Robos can mediate midline repulsion in Drosophila in the absence of robo.

Figure 3

A–G. Stage 16–17 Drosophila embryos stained with mAb 1D4 (anti-FasII).

A. Wild type embryo. FasII-positive axon pathways do not cross the midline.

B. In robo mutant embryos, axons from the medial FasII pathways cross the midline in every segment.

C–G. robo mutant embryos carrying elav-GAL4 and indicated UAS-Robo transgenes.

C. Pan-neural expression of Drosophila Robo fully rescues the robo loss of function phenotype: FasII-positive axons no longer cross the midline.

D,E. Expression of Drosophila Robo2 (D) or Robo3 (E) does not prevent ectopic midline crossing in a robo mutant background.

F,G. Expression of Tribolium Robo (D) or Tribolium Robo2/3 (E) is sufficient to prevent ectopic midline crossing in the absence of endogenous robo.

H. Quantification of ectopic midline crossing (average percent segments with ectopic FasII crossing per embryo) in the genetic backgrounds shown in A–G. Error bars represent s.e.m. Number of embryos scored for each genotype is indicated in parentheses.