. Author manuscript; available in PMC: 2014 Aug 11.

Published in final edited form as: Dev Biol. 2012 Jan 8;363(1):266–278. doi: 10.1016/j.ydbio.2011.12.046

Table 1.

Quantification of Tribolium Robo knockdown phenotypes.

genetic background	FasII-positive axons at midline^a	medial shift of intermediate pathway^b	dorsal lateral pathway defects^b
genetic background	FasII-positive axons at midline^a	medial shift of intermediate pathway^b	medial shift^c	missing
wild type	0.0 (n=48/8)	0.0 (n=93/8)	0.0 (n=95/8)	0.0 (n=95/8)
TcRobo RNAi	91.9 (n=74/11)	1.5 (n=131/11)	0.8 (n=119/11)	0.0 (n=119/11)
TcRobo2/3 RNAi	0.0 (n=51/10)	83.3 (n=102/10)	11.0 (n=100/10)	69.0 (n=100/10)

4–5 day old embryos were stained with anti-HRP and anti-TcFasII and abdominal segments were scored for defects in midline repulsion and longitudinal pathway formation. Percentage of segments or hemisegments displaying each phenotype are shown.

n = number of segments/number of embryos scored.

n = number of hemisegments/number of embryos scored.

hemisegments were scored as “medial shift” if axons could be seen transitioning from lateral to intermediate pathways within the hemisegment.