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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: Methods. 2014 May 2;68(1):218–227. doi: 10.1016/j.ymeth.2014.04.020

Table 1.

Antibodies and transgenic lines useful for studying spermatogenesis

Cell type
/structure
Marker Type Expression/Localization Source/References
Soma Fasciclin 3 (Fas3) Antibody Hub cells DSHB [130]
Armadillo Antibody Hub cells DSHB,[131]
upd-lacZ P-element insertion Hub cells [121, 132134]
esg-lacZ (M5–4) P-element insertion Hub cells [57]
esg-lacZ (esgG66B) P-element insertion Hub cells, cyst cells [57, 135]
Traffic Jam (Tj) Antibody CySCs and early cyst cells [136]
Zfh-1 Antibody CySCs and early cyst cells [19, 137]
Eyes Absent (Eya) Antibody Late cyst cells DSHB [138, 139]
esg-GFP P-element insertion Soma and germline [140]
Germline UASt-Stat-GFP GAL4/UAS GSCs [141, 142]
STAT92E Antibody GSCs [143]
GFP-Nanos Transgene GSCs and early germline cysts [144]
bam-GFP Transgene Mitotic germline cysts [144]
UASp-Bam-GFP GAL4/UAS Mitotic germline cysts [145]
Bam Antibody Mitotic germline cysts DSHB, [77]
Vasa-GFP Transgene Germline [146]
Vasa Antibody Germline Santa Cruz Biotechnology #sc-26877, [147]
Cytoskeleton UASp-E-Cadherin-GFP GAL4/UAS Cell adhesion [148]
UASp-Lifeact-GFP GAL4/UAS Actin, RCs BDSC #35544, [149]
UASp-Actin-GFP GAL4/UAS Actin, RCs [150]
GFP-Moesin Transgene Binds actin [151]
UASp-GFP-Tubulin GAL4/UAS Microtubules [152]
EB1-GFP Transgene Microtubule plus-ends [153]
UAS-IVS-myrtdTomato GAL4/UAS Plasma membranes BDSC #32223, [154]
α-Spectrin Antibody Fusome DSHB, [155]
Cytokinesis GFP-Anillin Transgene Contractile rings, RCs, nuclei [101]
mRFP-Anillin Transgene Contractile rings, RCs, nuclei [101]
Anillin Antibody Contractile rings, RCs, nuclei [102, 156]
GFP-Pavarotti Transgene Spindle midzone, RCs, nuclei [102, 157]
Pavarottii Antibody Spindle midzone, RCs [157]
Cindr Antibody Contractile rings and RCs [102, 156]
pTyr Antibody RCs SIGMA #T1325, Santa Cruz Biotechnology #sc-508, [68]
Zipper-GFP P-element insertion Contractile rings and RCs BDSC #51564, [102, 146]
Sqh-RFP Transgene Contractile rings and RCs [158]
Sqh-GFP Transgene Contractile rings and RCs [159]
Nucleus His2Av-mRFP Transgene Histones BDSC #23650 & #23651[160]
His2Av-EGFP Transgene Histones BDSC #24163, [161]
Sa-GFP Transgene Nucleolus in spermatocytes [162]
Polycomb-GFP Transgene Chromatin [162]