Table 1.
Antibodies and transgenic lines useful for studying spermatogenesis
| Cell type /structure |
Marker | Type | Expression/Localization | Source/References |
|---|---|---|---|---|
| Soma | Fasciclin 3 (Fas3) | Antibody | Hub cells | DSHB [130] |
| Armadillo | Antibody | Hub cells | DSHB,[131] | |
| upd-lacZ | P-element insertion | Hub cells | [121, 132–134] | |
| esg-lacZ (M5–4) | P-element insertion | Hub cells | [57] | |
| esg-lacZ (esgG66B) | P-element insertion | Hub cells, cyst cells | [57, 135] | |
| Traffic Jam (Tj) | Antibody | CySCs and early cyst cells | [136] | |
| Zfh-1 | Antibody | CySCs and early cyst cells | [19, 137] | |
| Eyes Absent (Eya) | Antibody | Late cyst cells | DSHB [138, 139] | |
| esg-GFP | P-element insertion | Soma and germline | [140] | |
| Germline | UASt-Stat-GFP | GAL4/UAS | GSCs | [141, 142] |
| STAT92E | Antibody | GSCs | [143] | |
| GFP-Nanos | Transgene | GSCs and early germline cysts | [144] | |
| bam-GFP | Transgene | Mitotic germline cysts | [144] | |
| UASp-Bam-GFP | GAL4/UAS | Mitotic germline cysts | [145] | |
| Bam | Antibody | Mitotic germline cysts | DSHB, [77] | |
| Vasa-GFP | Transgene | Germline | [146] | |
| Vasa | Antibody | Germline | Santa Cruz Biotechnology #sc-26877, [147] | |
| Cytoskeleton | UASp-E-Cadherin-GFP | GAL4/UAS | Cell adhesion | [148] |
| UASp-Lifeact-GFP | GAL4/UAS | Actin, RCs | BDSC #35544, [149] | |
| UASp-Actin-GFP | GAL4/UAS | Actin, RCs | [150] | |
| GFP-Moesin | Transgene | Binds actin | [151] | |
| UASp-GFP-Tubulin | GAL4/UAS | Microtubules | [152] | |
| EB1-GFP | Transgene | Microtubule plus-ends | [153] | |
| UAS-IVS-myrtdTomato | GAL4/UAS | Plasma membranes | BDSC #32223, [154] | |
| α-Spectrin | Antibody | Fusome | DSHB, [155] | |
| Cytokinesis | GFP-Anillin | Transgene | Contractile rings, RCs, nuclei | [101] |
| mRFP-Anillin | Transgene | Contractile rings, RCs, nuclei | [101] | |
| Anillin | Antibody | Contractile rings, RCs, nuclei | [102, 156] | |
| GFP-Pavarotti | Transgene | Spindle midzone, RCs, nuclei | [102, 157] | |
| Pavarottii | Antibody | Spindle midzone, RCs | [157] | |
| Cindr | Antibody | Contractile rings and RCs | [102, 156] | |
| pTyr | Antibody | RCs | SIGMA #T1325, Santa Cruz Biotechnology #sc-508, [68] | |
| Zipper-GFP | P-element insertion | Contractile rings and RCs | BDSC #51564, [102, 146] | |
| Sqh-RFP | Transgene | Contractile rings and RCs | [158] | |
| Sqh-GFP | Transgene | Contractile rings and RCs | [159] | |
| Nucleus | His2Av-mRFP | Transgene | Histones | BDSC #23650 & #23651[160] |
| His2Av-EGFP | Transgene | Histones | BDSC #24163, [161] | |
| Sa-GFP | Transgene | Nucleolus in spermatocytes | [162] | |
| Polycomb-GFP | Transgene | Chromatin | [162] |