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. Author manuscript; available in PMC: 2015 May 8.
Published in final edited form as: Cell Rep. 2014 May 1;7(3):722–734. doi: 10.1016/j.celrep.2014.04.025

Figure 3. Esg is required autonomously in hub cells to maintain the stem cell niche.

Figure 3

(A, C) DIC images of RNA in situ for upd in 1-day old wild-type (A) or esgshof (C) flies. (B, D) Testes from 1-day old cdi-lacZ (B) or esgshof; cdi-lacZ (D) flies immunostained for Fas3 (blue), DE-cadherin (DE-Cad, red, insets), β-gal (green). Phase contrast image of testes from 10-day old updGal4, UAS-GFP;Gal80ts (E) or updGal4;UAS-esgRNAi/Gal80ts (G) flies shifted to 29°C upon eclosion to induce transgene expression. Immunofluorescence images of testes from 10-day old updGal4, UAS-GFP (F) and updGal4, UAS-GFP; UAS-esgRNAi (H) males for GFP (green, hub), TJ (red) and DAPI shifted to 29°C upon eclosion. Scale bars, 10 μM.