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. Author manuscript; available in PMC: 2015 Jul 24.
Published in final edited form as: Cell Rep. 2014 Jul 10;8(2):371–381. doi: 10.1016/j.celrep.2014.06.025

Figure 5. YAP activation is sufficient to revert the cell cycle arrest in TKO livers.

Figure 5

(A–C) Immunofluorescence analysis of YFP (green) together with endogenous YAP (A), MCM6 (B), or Ki67 (C), respectively, after 2/3rd partial hepatectomy (PH) 2 weeks after Tam in CreER transposon-injected cTKO;RosaLSL-YFP mice. (D) Immunofluorescence staining of GFP (green) and Ki67 (red) 5 days after 2/3rd PH, 2 weeks after Tam in CreER transposon-injected cTKO;RosamTmG mice. (E) Immunofluorescence analysis of YAP (Flag, green) and Ki67 (red) following hydrodynamic tail vein injection of a transposon expressing either wild type YAP or constitutively active variants of YAP (YAPS127A, YAP3SA, or YAP5SA) into control or arrested TKO livers (transposon injection 1 week after 50 µg Tam, livers analyzed 2 weeks after Tam). (F) Quantification of Ki67 positive YAP-transfected hepatocytes. Data represented as mean +/− SD. (G) Quantification of MCM6 positive hepatocytes following YAP3SA expression in controls or arrested TKO livers (transposon injection 1 week after 50 µg Tam, livers analyzed 2 weeks after Tam). Controls, Flag negative cells in control livers; TKO, Flag negative cells in arrested TKO livers; YAP3SA, Flag positive cells in control livers; YAP3SA TKO, Flag positive cells in arrested TKO livers.