Table 1.
Primer-probe sets used for normalized, relative quantitative PCR for the genes under study. Pre-designed TaqMan® gene expression assays from Applied Biosystems® were used to quantify gene expression. To minimize the influence of residual genomic DNA contamination, genomic DNA elimination using the QIAGEN method during RNA isolation. Additionally, where possible, primer-probe sets spanning multiple exons were used to further reduce potential for genomic DNA interference.
| Gene ID | Gene function | Gene location | Assay ID |
|---|---|---|---|
| CYP1A1 | Phase I metabolism | 15q24.1 | Hs00153120_m1 |
| CYP1B1 | 2p22.2 | Hs00164383_m1 | |
| EPHX1 | 1q42.12 | Hs01116806_m1 | |
| GSTT1 | Phase II metabolism | 22q11.23 | Hs00184475_m1 |
| GSTM1 | 1p13.3 | Hs02341469_m1 | |
| XPA | DNA damage repair | 9q22.33 | Hs00166045_m1 |
| MT1A | Metal binding | 16q12.2 | Hs00831826_s1 |
| MT2A | 16q12.2 | Hs02379661_g1 | |
| ACTB | Reference | 7p22.1 | Hs99999903_m1 |