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. Author manuscript; available in PMC: 2015 Jul 8.
Published in final edited form as: Anal Methods. 2014 May 13;6(15):5427–5449. doi: 10.1039/C4AY00447G

Table 2.

CGE and MGE

Analyte Mode Gel Capillary Voltage Detection Notes Ref.
In-house IgG2λ,
IgG2K, and
IgG1K mAbs
Nonreducing Beckman Coulter
SDS-MW gel buffer
30 (20) cm,
50 µm id
15 kV UV 220 nm Monitor disulfide reduction
during production
[36]
In-house IgG1
mAbs
Reducing and
nonreducing
Agilent High Sensitivity
Protein 250 Kit
Agilent 2100
Bioanalyzer
NR LIF Characterization of size
variants
[37]
In-house IgG1
and IgG4 mAbs
Reducing and
nonreducing
Beckman Coulter
SDS-MW gel buffer
31.2 (20) cm,
50 µm id
−15 kV LIF ex. 488 nm /
em. 600 nm
Impurity analysis [38]
IgG 1 mAb Reducing Beckman Coulter
SDS-MW gel buffer
30.2 (20) cm,
50 µm id
−15kV PDA Comparison of SDS-CGE
To SDS-PAGE
[39]
Myoglobin, carbonic
anhydrase I,
ovalbumin, BSA
Nonreducing Beckman Coulter
SDS-MW gel buffer
33 (24.5) cm,
50 µm id
−16.5 kV 220 nm Demonstration of
improved precision
[40]
In-house Fc-fusion
proteins, and IgG1
and IgG2 mAbs
Reducing and
nonreducing
Beckman Coulter
SDS-MW gel buffer
30 (20) cm,
50 µm id
15 kV 220 nm Automated sample
preparation
[41]
In-house vaccine
proteins
Reducing Protein Simple
separation matrix
12-capillary cartridge;
5 cm, 100 µm id
250 V Chemiluminescence
from secondary
antibody
Automated separation and
Western blot
[42]
Ricin A-chain
immunotoxins
Reducing and
nonreducing
Bio-Rad CE-SDS
run buffer
24 (19.5) cm,
50 µm id
5 or 15 kV UV 220 nm and
MALDI-TOF-MS
CGE-MALDI-TOF-MS of
ricin proteins
[45]
In-house mAbs and
proteins
MGE
Reducing and
nonreducing
HT Protein Express
gel matrix
LabChip GXII NR Indirect and direct LIF
ex. 620 nm /
em. 700 nm
MGE methods for high-
resolution and high-
sensitivity
[138]
Actin, carbonic,
anhydrase II,
and, lysozyme
MGE
Nonreducing
Beckman Coulter
SDS-MW gel buffer
2 cm glass microchip 480 kV Western blot MGE separation with off-
chip Western blot detection
[140]

Capillary: actual length (effective length), inner diameter

Bovine serum albumin (BSA), Not reported (NR)