Table 2.

CGE and MGE

Analyte	Mode	Gel	Capillary	Voltage	Detection	Notes	Ref.
In-house IgG2λ, IgG2K, and IgG1K mAbs	Nonreducing	Beckman Coulter SDS-MW gel buffer	30 (20) cm, 50 µm id	15 kV	UV 220 nm	Monitor disulfide reduction during production	[36]
In-house IgG1 mAbs	Reducing and nonreducing	Agilent High Sensitivity Protein 250 Kit	Agilent 2100 Bioanalyzer	NR	LIF	Characterization of size variants	[37]
In-house IgG1 and IgG4 mAbs	Reducing and nonreducing	Beckman Coulter SDS-MW gel buffer	31.2 (20) cm, 50 µm id	−15 kV	LIF ex. 488 nm / em. 600 nm	Impurity analysis	[38]
IgG 1 mAb	Reducing	Beckman Coulter SDS-MW gel buffer	30.2 (20) cm, 50 µm id	−15kV	PDA	Comparison of SDS-CGE To SDS-PAGE	[39]
Myoglobin, carbonic anhydrase I, ovalbumin, BSA	Nonreducing	Beckman Coulter SDS-MW gel buffer	33 (24.5) cm, 50 µm id	−16.5 kV	220 nm	Demonstration of improved precision	[40]
In-house Fc-fusion proteins, and IgG1 and IgG2 mAbs	Reducing and nonreducing	Beckman Coulter SDS-MW gel buffer	30 (20) cm, 50 µm id	15 kV	220 nm	Automated sample preparation	[41]
In-house vaccine proteins	Reducing	Protein Simple separation matrix	12-capillary cartridge; 5 cm, 100 µm id	250 V	Chemiluminescence from secondary antibody	Automated separation and Western blot	[42]
Ricin A-chain immunotoxins	Reducing and nonreducing	Bio-Rad CE-SDS run buffer	24 (19.5) cm, 50 µm id	5 or 15 kV	UV 220 nm and MALDI-TOF-MS	CGE-MALDI-TOF-MS of ricin proteins	[45]
In-house mAbs and proteins	MGE Reducing and nonreducing	HT Protein Express gel matrix	LabChip GXII	NR	Indirect and direct LIF ex. 620 nm / em. 700 nm	MGE methods for high- resolution and high- sensitivity	[138]
Actin, carbonic, anhydrase II, and, lysozyme	MGE Nonreducing	Beckman Coulter SDS-MW gel buffer	2 cm glass microchip	480 kV	Western blot	MGE separation with off- chip Western blot detection	[140]

Capillary: actual length (effective length), inner diameter

Bovine serum albumin (BSA), Not reported (NR)