Figure 6. Requirement of LXR receptors during niche modulation.
(A) Relative levels of Mertk and Abca1 gene expression at different Zeitgeber times of the day. For reference, the dotted line shows the levels of blood neutrophils. Shaded areas represent periods of darkness. n=4–9 mice per time.
(B) Levels of Cxcl12 and Abca1 transcripts in BM and number of blood CFU-C after treatment with the LXR agonist GW3965, or with vehicle control (DMSO). n=5 mice.
(C) Number of blood CFU-C and CXCL12 protein levels in the BM of WT or LXR-deficient mice (LXR dKO) after treatment with saline or 106 neutrophils. Samples were analyzed at ZT1. n=7–9 mice.
Data are shown as mean ± s.e.m. *P<0.05, **P<0.01, ***P<0.001. See also Figure S5.