TABLE 1 .
Stability of the insertions in NH-2 recombinants, following passages in cells
| Parental virus name | Type | Recombination site | Insertion length, nt | Preservation of insertion |
||||
|---|---|---|---|---|---|---|---|---|
| Duplicate | Status at passage no.a: |
Recombination site | ||||||
| P8 | P13 | P18 | ||||||
| S2CA17Δ cl 9.2 | NH-2 | S2-3401 (2A) | 8 | A | Yes | Yes | Yes | Parentalb |
| CA17-3396 (VP1) | B | Yes | Yes | Yes | Parental | |||
| S2CA17 cl 1.1 | NH-2 | S2-3454 (2A) | 86 | A | No | S2-3407 (2A)c | ||
| CA17-3215 (VP1) | B | Yes | Yes | Yes | Parental | |||
| S2CA17Δ cl 11.1 | NH-2 | S2-3622 (2A) | 101 | A | Yes | No | S2-3458 (2A) | |
| CA17-3338 (VP1) | B | No | S2-3501 (2A) | |||||
| S2CA17 cl 2.1 | NH-1 | S2-3840 (2B) | 3 | A | Yes | Yes | Yes | Parental |
| CA17-3850 (2B) | B | Yes | Yes | Yes | Parental | |||
| S2CA17Δ cl 8.1 | NH-2 | S2-5125 (3A) | 87 | A | No | S2-5003 (2C) | ||
| CA17-4883 (2C) | B | No | S2-5060 (2C) | |||||
| S2CA17 cl 4.4 | NH-2 | S2-5209 (3A) | 70 | A | Yes | Yes | Md | Mixtured |
| CA17-5018 (2C) | B | Yes | Yes | M | Mixture | |||
| S2CA17Δ cl 12.2 | NH-2 | S2-5214 (3A) | 49 | A | Yes | Yes | Yes | Parental |
| CA17-5086 (2C) | B | Yes | Yes | Yes | Parental | |||
a
Number of passages in cells. Preservation or loss of insertion (duplicated homologous sequences) is indicated, following passages.
b
Recombination site unchanged, identical to that in the original virus.
c
Homologous recombination site: nucleotide position in the Sabin 2 genome is indicated.
d
Indicates a mixture of NH and H recombinants.