TABLE 2 .
Stability of the insertions in NH recombinants, following the inoculation of mice
| Inoculation route | Virus | Type | Recombination site | Insertion length, nt | Proportion of sick micea | Stabilityb |
|---|---|---|---|---|---|---|
| Intranasal | MADCA17Δ cl 19 | NH-2 | S2-3407 (2A) | 58 | 3/5 | Yes |
| CV-A17-3252 (VP1) | ||||||
| MADCA17Δ cl 28 | NH-1 | S2-3426 (2A) | 5 | 4/5 | Yes | |
| CV-A17-3430 (2A) | ||||||
| MADCA17Δ cl 1 | NH-2 | S2-3889 (2B) | 91 | 0/5 | NAc | |
| CV-A17-3635 (2A) | ||||||
| MADCA17Δ cl a37 | NH-1 | S2-3849 (2B) | 4 | 4/5 | Yes | |
| CV-A17-3856 (2B) | ||||||
| Intraperitoneal | MADCA17Δ cl 19 | NH-2 | S2-3407 (2A) | 58 | 4/6 | Yes |
| CV-A17-3252 (VP1) | ||||||
| MADCA17Δ cl 28 | NH-1 | S2-3426 (2A) | 5 | 3/6 | Yes | |
| CV-A17-3430 (2A) | ||||||
| MADCA17Δ cl 1 | NH-2 | S2-3889 (2B) | 91 | 3/6 | Yes | |
| CV-A17-3635 (2A) | ||||||
| MADCA17Δ cl a37 | NH-1 | S2-3849 (2B) | 4 | 3/6 | Yes | |
| CV-A17-3856 (2B) | ||||||
| Intracerebral | MADCA17Δ cl 1 | NH-2 | S2-3889 (2B) | 91 | 5/6 | Nod |
| CV-A17-3635 (2A) |
a
Number of paralyzed or dead mice/number of animals inoculated. Regarding the inoculated doses and the inoculation routes (Materials and Methods), the nonpathogenic S2 and the neurovirulent S2/4568 or MAD04 virus paralyzed or killed 0% and >75% of inoculated mice, respectively.
b
Stability of the insertions in viruses isolated from the medulla of paralyzed or dead mice.
c
NA, not applicable.
d
Excision of the insertion leading to homologous recombinants in the medulla of 3 of 5 paralyzed or dead mice.