Figure 4.
Ethidium bromide−stained agarose gels comparing disproportionate ratios of Anopheles gambiae and An. stephensi mixed templates in the absence of the universal forward primer UN400F. A, Constant concentration of An. stephensi template mixed with serial dilutions of An. gambiae template. B, Constant concentration of An. gambiae template mixed with serial dilutions of An. stephensi template. An. stephensi (lane 1), An. gambiae (lane 2), mixed 1:1 (equal concentrations of both templates, lane 3), 1:2 (lane 4), 1:4 (lane 5), 1:8 (lane 6), 1:16 (lane 7), 1:32 (lane 8), 1:64 (lane 9), 1:128 (lane 10), 1:256 (lane 11), negative control (lane 12), 100-basepair (bp)-molecular marker (lanes L).