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. Author manuscript; available in PMC: 2015 Jul 31.
Published in final edited form as: Nature. 2014 Jul 13;511(7511):551–556. doi: 10.1038/nature13442

Figure 3. CO interference requires post-translational modification.

Figure 3

a–c, WT and mutant CO patterns (black; colors). Quantitatively similar decreases in CO interference and increases in CO number are seen in: ubc9-GFP (SUMO E2; brown); red1KR (non-SUMOylated Red1; cyan); strains lacking Slx5 or Slx8 (slx5Δ or slx8Δ) or mutated for the Slx5 SUMO-binding motif or the Slx8 ubiquitin ligase motif (slx5-SIM, slx8-SS) (magenta); or lacking Sir2 (sir2Δ) or mutated for the Sir2/Slx5 interaction site (sir2RK) (blue). CO interference does not require Sir2 deacetylation activity (sir2-345) or Sir2 interaction partner Sir4 (sir4Δ) (grey) or other Sir2 activities/partners (text).