Table 17.4.1.
Crystallization Matrix Components Used in Crystal Screen 1 (Hampton Research) to Crystallize Proteins
| Precipitant | Salt | Buffer |
|---|---|---|
| Ammonium phosphate | Ammonium acetate | Imidazole, pH 6.5 |
| Ammonium sulfate | Ammonium sulfate | Sodium acetate, pH 4.6 |
| Lithium sulfate | Calcium acetate | Sodium citrate, pH 5.6 |
| Magnesium formate | Calcium chloride | Sodium cacodylate, pH 6.5 |
| MPDa | Lithium sulfate | Sodium HEPES, pH 7.5 |
| PEGb (400, 1500, 4000, 8000) | Magnesium acetate | Tris·Cl, pH 8.5 |
| Potassium phosphate | Magnesium chloride | |
| Potassium tartrate | Sodium acetate | |
| 2-Propanol | Sodium citrate | |
| Sodium acetate | Zinc acetate | |
| Sodium citrate | ||
| Sodium formate | ||
| Sodium phosphate | ||
| Sodium tartrate |
a
MPD, 2-methyl-2,4-pentanediol.
b
PEG, polyethylene glycol.