Table 17.4.4.
Components of Natrix (Hampton Research) for Nucleic Acid and Protein-Nucleic Acid Complexes Screena
| Condition | Formulationb |
|---|---|
| 1 | 0.01 M magnesium chloride, 0.05 M MES, pH 5.6, 1.8 M lithium sulfate |
| 2 | 0.01 M magnesium acetate, 0.05 M MES, pH 5.6, 2.5 M ammonium sulfate |
| 3 | 0.1 M magnesium acetate, 0.05 M MES, pH 5.6, 20% MPD |
| 4 | 0.2 M KCl, 0.01 M magnesium sulfate, 0.05 M MES, pH 5.6, 10% PEG 400 |
| 5 | 0.2 M KCl, 0.01 M magnesium chloride, 0.05 M MES, pH 5.6, 5% PEG 8000 |
| 6 | 0.1 M ammonium sulfate, 0.01 M magnesium chloride, 0.05 M MES, pH 5.6, 20% PEG 8000 |
| 7 | 0.02 M magnesium chloride, 0.05 M MES, pH 6.0, 15% isopropanol |
| 8 | 0.005 M magnesium sulfate, 0.1 M ammonium acetate, 0.05 M MES, pH 6.0, 0.6 M NaCl |
| 9 | 0.1 M KCl, 0.01 M magnesium chloride, 0.05 M MES, pH 6.0, 10% PEG 400 |
| 10 | 0.005 M magnesium sulfate, 0.05 M MES, pH 6.0, 5% PEG 4000 |
| 11 | M magnesium chloride, 0.05 M sodium cacodylate, pH 6.0, 1.0 M lithium sulfate |
| 12 | M magnesium sulfate, 0.05 M sodium cacodylate, pH 6.0, 1.8 M lithium sulfate |
| 13 | 0.015 M magnesium acetate, 0.05 M sodium cacodylate, pH 6.0, 1.7 M ammonium sulfate |
| 14 | M KCl, 0.025 M magnesium chloride, 0.05 M sodium cacodylate, pH 6.0, 15% isopropanol |
| 15 | 0.04 M magnesium chloride, 0.05 M sodium cacodylate, pH 6.0, 5% MPD |
| 16 | 0.04 M magnesium acetate, 0.05 M sodium cacodylate, pH 6.0, 30% MPD |
| 17 | M KCl, 0.01 M calcium chloride, 0.05 M sodium cacodylate, pH 6.0, 10% PEG 4000 |
| 18 | M magnesium acetate, 0.05 M sodium cacodylate, pH 6.5, 1.3 M lithium sulfate |
| 19 | M magnesium sulfate, 0.05 M sodium cacodylate, pH 6.5, 2.0 M ammonium sulfate |
| 20 | M ammonium acetate, 0.015 M magnesium acetate, 0.05 M sodium cacodylate, pH 6.5, 10% isopropanol |
| 21 | 0.2 M KCl, 0.005 M magnesium chloride, 0.05 M sodium cacodylate, pH 6.5, 10% 1,6-hexanediol |
| 22 | 0.08 M magnesium acetate, 0.05 M sodium cacodylate, pH 6.5 |
| 23 | M KCl, 0.01 M magnesium chloride, 0.05 M sodium cacodylate, pH 6.5, 10% PEG 4000 |
| 24 | 0.2 M ammonium acetate, 0.01 M calcium chloride, 0.05 M sodium cacodylate, pH 6.5, 10% PEG 4000 |
| 25 | 0.08 M magnesium acetate, 0.05 M sodium cacodylate, pH 6.5, 30% PEG 4000 |
| 26 | M KCl, 0.1 M magnesium acetate, 0.05 M sodium cacodylate, pH 6.5, 10% PEG 8000 |
| 27 | M ammonium acetate, 0.01 M magnesium acetate, 0.05 M sodium cacodylate, pH 6.5, 30% PEG 8000 |
| 28 | 0.05 M magnesium sulfate, 0.05 M sodium HEPES, pH 7.0, 1.6 M lithium sulfate |
| 29 | 0.01 M magnesium chloride, 0.05 M sodium HEPES, pH 7.0, 4.0 M lithium chloride |
| 30 | 0.01 M magnesium chloride, 0.05 M sodium HEPES, pH 7.0 |
| 31 | 0.005 M magnesium chloride, 0.05 M sodium HEPES, pH 7.0, 25% PEG monomethyl ether 550 |
| 32 | 0.2 M KCl, 0.01 M magnesium chloride, 0.05 M sodium HEPES, pH 7.0, 20% 1,6- hexanediol |
| 33 | 0.2 M ammonium chloride, 0.01 M magnesium chloride, 0.05 M sodium HEPES, pH 7.0, 30% 1,6-hexanediol |
| 34 | 0.1 M KCl, 0.005 M magnesium sulfate, 0.05 M sodium HEPES, pH 7.0, 15% MPD |
| 35 | M KCl, 0.01 M magnesium chloride, 0.05 M sodium HEPES, pH 7.0, 5% PEG 400 |
| 36 | 0.1 M KCl, 0.01 M calcium chloride, 0.05 M sodium HEPES, pH 7.0, 10% PEG 400 |
| 37 | M KCl, 0.025 M magnesium sulfate, 0.05 M sodium HEPES, pH 7.0, 20% PEG 200 |
| 38 | 0.2 M ammonium acetate, 0.15 M magnesium acetate, 0.05 M sodium HEPES, pH 7.0, 5% PEG 4000 |
| 39 | M ammonium acetate, 0.02 M magnesium chloride, 0.05 M sodium HEPES, pH 7.0, 5% PEG 8000 |
| 40 | 0.01 M magnesium chloride, 0.05 M Tris·Cl, pH 7.5, 1.6 M ammonium sulfate |
| 41 | M KCl, 0.015 M magnesium chloride, 0.05 M Tris·Cl, pH 7.5, 10% PEG monomethyl ether 550 |
| 42 | 0.01 M magnesium chloride, 0.05 M Tris·Cl, pH 7.5, 5% isopropanol |
| 43 | M magnesium chloride, 0.05 M ammonium acetate, 0.05 M Tris·Cl, pH 7.5, 10% MPD |
| 44 | 0.2 M KCl, 0.05 M magnesium chloride, 0.05 M Tris·Cl, pH 7.5, 10% PEG 4000 |
| 45 | 0.025 M magnesium sulfate, 0.05 M Tris·Cl, pH 8.5, 1.8 M ammonium sulfate |
| 46 | 0.005 M magnesium sulfate, 0.05 M Tris·Cl, pH 8.5, 35% 1,6-hexanediol |
| 47 | 0.1 M KCl, 0.01 M magnesium chloride, 0.05 M Tris·Cl, pH 8.5, 30% PEG 400 |
| 48 | M calcium chloride, 0.2 M ammonium chloride, 0.05 M Tris·Cl, pH 8.5, 30% PEG 4000 |
b
MES, 2-(N-morpholino)ethanesulfonic acid; MPD, 2-methyl-2,4-pentanediol; PEG, polyethylene glycol.