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. Author manuscript; available in PMC: 2014 Dec 5.
Published in final edited form as: Nature. 2014 Apr 13;510(7503):152–156. doi: 10.1038/nature13279

Extended Data Fig. 1. Stimulation of SILP Th17 cells requires intestinal microbiota antigen presentation.

Extended Data Fig. 1

(a) Intestinal GFP+ CD4+ T cells from Il-23rGFP/+ mice stimulated with fecal material from Jax and Tac mice in the presence of syngeneic splenic APCs. Forward scatter was evaluated after 2 days. (b) Th17 cell activation by fecal material from SFB-monoassociated mice in the presence of APCs sufficient (WT) or deficient (KO) for MHC class II. (c) Evaluation of potential activation of bystander CD4+ T cells upon stimulation with SFB antigen. SILP CD4+ T cells from mice with Jax flora (Ly5.1) and Taconic flora (Ly5.2) were co-cultured or stimulated separately with APCs and SFB-monoassociated fecal material, and FSC was evaluated.