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. Author manuscript; available in PMC: 2014 Dec 5.
Published in final edited form as: Nature. 2014 Apr 13;510(7503):152–156. doi: 10.1038/nature13279

Extended Data Fig. 4. Single-cell TCR cloning and TCR hybridoma screen.

Extended Data Fig. 4

(a) Efficiency of single-cell Vβ14 cloning from SILP Th17 and non-Th17 cells of multiple mice. (b) Distributions of unique Vβ14 sequences in Th17 and non-Th17 cells within the SILP. Each plot represents one mouse shown in (a). y and x axes represent numbers of Th17 cells and non-Th17 cells for each unique Vβ14 sequence. Numbers of unique sequences are shown in colored circles. (c) Responses of Th17 and non-Th17 TCR hybridomas to small intestinal luminal contents from B6 Tac and B6 Jax mice. (d) Stimulation of Th17 TCR hybridomas by SFB-monoassociated antigens in the presence of APCs sufficient (WT) or deficient (KO) for MHC class II.