Extended Data Fig. 6. Identification of SFBNYU_004990 epitopes recognized by related TCRs.
(a) Top: The distribution in Th17 and non-Th17 cells of four TCRs that share an identical TCRα chain. Bottom: Amino acid alignment of the Vβ14 CDR3 sequences. The green box highlights the sequence differences. (b) Stimulation of the 5A11 hybridoma by bacterial pool 2D10 in the SFB antigen screen. (c) Responses of 4 TCR hybridomas, including a non-Th17 hybridoma, to bacterial clone 2D10-A10. (d) Responses of the 2D10-A10-specific TCR hybridomas to core epitopes encoded by minigenes expressed in E.coli. (e) TCR hybridoma responses to titrated synthetic peptide (IRWFGSSVQKV) in the presence of APCs.