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. Author manuscript; available in PMC: 2014 Dec 5.
Published in final edited form as: Nature. 2014 Apr 13;510(7503):152–156. doi: 10.1038/nature13279

Extended Data Fig. 7. SFB epitopes recognized by diverse Th17 cell TCRs.

Extended Data Fig. 7

(a) The epitopes recognized by the Vβ14+ TCR hybridomas stimulate only Vβ14+ Th17 cells from the SILP. Th17 cells sorted from Il-23rGFP/+ mice were stimulated with indicated peptides (listed in (d)) in the presence of APCs. Left: Representative IL-17A ELISPOT assay with triplicates. Right: Normalized peptide-specific Th17 responses. Each dot represents one mouse. (b) Polyclonal responses of Vβ14+ and Vβ14- SILP Th17 cells to SFB antigens. Representative FACS plots from five experiments are shown. (c) Bioinformatics filtering approach to select candidate SFB epitopes. (d) Summary of newly-selected and the known A6 and A15 SFB peptides. (e) IL-17A ELISPOT screen for indicated peptides using SILP Th17 cells sorted from SFB-colonized Il-23rGFP/+ mice. The A6 peptide from SFBNYU_003340 and anti-CD3 served as positive controls. (f) Vβ14 usage in Th17 cells specific for peptide N5. Left: Representative IL-17A ELISPOT assay with triplicates for peptide N5, using Vβ14+ and Vβ14- SILP Th17 cells sorted from Il-23rGFP/+ mice. Right: Normalized N5-specific Th17 responses. Each dot represents one mouse.