Extended Data Fig. 9. Transcription factor expression in SFB-specific and Listeria-specific T cells in co-infected mice (representative of data plotted in Fig. 4b).
(a) Experimental design for tracking both SFB- and Listeria- specific CD4+ T cells following intestinal colonization with both bacteria. Ly5.2 B6 mice were colonized with Listeria monocytogenes, SFB, or both bacteria, and 7B8Tg T cells from Ly5.1 mice were injected IV. Expression of Th1 and Th17 transcription factors in the SFB-specific 7B8Tg cells and LLO tetramer-specific recipient T cells was evaluated. (b) Intracellular stain for RORγt. (c) Intracellular stain for T-bet.