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. 2014 Jul 23;11:125. doi: 10.1186/1742-2094-11-125

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Copyright © 2014 Lisi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of mammalian target of rapamycin (mTOR) inhibitors on microglial proliferation. Microglia cells were treated for 48 h with control-conditioned medium (C-CM) (A) or CM from glioma cells activated with lipopolysaccharide (LPS)/IFNγ (LI-CM) (B) and mTOR inhibitors. Effects on cell viability were assessed by the MTS reduction assay, while the effects on cell proliferation were assessed by 5-bromo-2-deoxyuridine (BrDU) incorporation. Data were analyzed by one-way analysis of variance followed by Bonferroni post hoc test.