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Frequency of T7dsp during growth in long term biofilms. The phage inoculum was T7dsp 3.8P. Each color-texture line combination represents a different biofilm, each inoculated with the same phage stock; the frequency of T7dsp in that stock is used as the initial frequency for all 7 adaptations. The black dotted line is for cells sloughed from a 7-day biofilm and shunted into a biofilm-free tube two hours before phage addition; those cells should be almost entirely planktonic.
