Table 2.
Phage evolution in long term biofilms 1
| Initial phages 2 | Final dsp frequency 3 | Confidence interval 4 |
|---|---|---|
| T7dsp |
1.0 |
(0.94, 1) |
| T7dsp |
1.0 |
(0.94, 1) |
| T7dsp, T7v |
0.15 |
(0.07, 0.33) |
| T7dsp, T7v |
0.0 |
(0, 0.06) |
| T7dsp, T7+ |
0.5 |
(0.24, 0.76) |
| T7dsp, T7+ | 0.15 | (0.07, 0.33) |
1The biofilms assayed here are a subset of those shown in Figures 5 and 6. 2T7dsp is T7dsp+trx 10B . Where two phages were inoculated, the starting frequencies were approximately 0.5 each. 3After 96hr phage growth, frequencies were estimated from 8 PCR reactions per sample for each of two primer sets, each reaction comprised of 6 plaque isolates. The PCR product from one primer set indicated presence of the dispersin gene in at least one of the 6 isolates of the reaction; the product from the other set indicated its absence in at least one of the 6 isolates. Frequency was estimated as the maximum likelihood value of the corresponding multinomial term. 4The confidence interval was calculated as the Bayesian credible interval using a uniform prior.