Table 4.
Bacterial strains
| Notation | Genotype: | Use | Ref |
|---|---|---|---|
| IJ1133 |
E. coli K-12 F -ΔlacX74 thi Δ(mcrC-mrr)102::Tn10 |
Host for measuring phage titers and some serial transfers to determine the evolutionary stability of the dsp B transgene in the absence of an extracellular PNAG matrix. |
[31] |
| BL21 (DE3) |
E. coli B Gal -dcm ompT hsdS(r
B
- m
B
-) [ λ(DE3)] |
Host for protein expression |
[32] |
| IJ512 |
E. coli K12 ΔlacX74 supE44 galK2 galT22 mcrA rfbD1 mcrB1 hsdS3 /F ′lac |
Host for selecting T7 carrying T3 gene 1.2 |
|
|
ΔtrxA |
JW5856 (Keio ΔtrxA::kan) |
Host for selection of positive phage recombinants carrying trxA |
KEIO [33] |
|
ΔcsrA |
E. coli K-12 F -ΔlacU169 ΔcsrA::kan |
Biofilm host; overproduces PNAG, the substrate for dispersin B |
TR1-5 mutant in [34] |
| ΔnagZ | JW1083 (Keio ΔnagZ::kan) | Host with low background activity for producing dispersin B protein from phage lysates | KEIO [33] |