Real-time Genomic PCR Primers and Probes |
|
p16: |
5’-ACTGCGATGTCGTACAGTATCTA-3’ (forward) |
5’-AGCCCTCAGATTTCAAACTCTT-3’ (reverse) |
5’-FAM-TAACTTCTGCCCAGACACCCCCA-TAMRA-3’ (probe) |
α-CMHC: |
5’-ACCCAGGGCTTCATGCGTAT-3’ (forward) |
5’-CCGAGATAGGGAGCTAGGCA-3’ (reverse) |
5’-TET-CAAGCGTCTACCAGCGAGCTCCAGG-TAMRA-3’ (probe) |
|
Methylation-specific PCR Primers |
|
Modified/Methylated: |
5’-GCGGTTGTTTAGGGTCGC-3’ (forward) |
5’-CTACCTAAATCGAAATACGACCG-3’ (reverse) |
Modified/Unmethylated: |
5’-GGAGTAGTATGGAGTTTTTTGTGGAT-3’ (forward) |
5’-TATACCTAAATCAAAATACAACCA-3’ (reverse) |
|
Cold SSCP Amplification PCR Primers |
|
Exon 1α |
5’-ATGGAGCCCTCTGCGGACG-3’ (forward) |
5’-GATGAGATACCCTGTGCCTAC-3’ (reverse) |
Exon 2a |
5’-AGCGGCAGTCTGGCCTTGAT-3’ (forward) |
5’-GCCCCCGCCTGGTGTAGTAT-3’ (reverse) |
Exon 2b |
5’-CGGGAGGGCTTCTTGGAAAC-3’ (forward) |
5’-TCAGGAGCCCTCAGATTTCA-3’ (reverse) |