Table 1.

PCR Primers and Probes.

Real-time Genomic PCR Primers and Probes

p16:

5’-ACTGCGATGTCGTACAGTATCTA-3’ (forward)

5’-AGCCCTCAGATTTCAAACTCTT-3’ (reverse)

5’-FAM-TAACTTCTGCCCAGACACCCCCA-TAMRA-3’ (probe)

α-CMHC:

5’-ACCCAGGGCTTCATGCGTAT-3’ (forward)

5’-CCGAGATAGGGAGCTAGGCA-3’ (reverse)

5’-TET-CAAGCGTCTACCAGCGAGCTCCAGG-TAMRA-3’ (probe)

Methylation-specific PCR Primers

Modified/Methylated:

5’-GCGGTTGTTTAGGGTCGC-3’ (forward)

5’-CTACCTAAATCGAAATACGACCG-3’ (reverse)

Modified/Unmethylated:

5’-GGAGTAGTATGGAGTTTTTTGTGGAT-3’ (forward)

5’-TATACCTAAATCAAAATACAACCA-3’ (reverse)

Cold SSCP Amplification PCR Primers

Exon 1α

5’-ATGGAGCCCTCTGCGGACG-3’ (forward)

5’-GATGAGATACCCTGTGCCTAC-3’ (reverse)

Exon 2a

5’-AGCGGCAGTCTGGCCTTGAT-3’ (forward)

5’-GCCCCCGCCTGGTGTAGTAT-3’ (reverse)

Exon 2b

5’-CGGGAGGGCTTCTTGGAAAC-3’ (forward)

5’-TCAGGAGCCCTCAGATTTCA-3’ (reverse)