Table 1.
Purification of SAS-1 and SAS-2 from 200 g of Victorin-Treated Leaves
| Purification Step | Total Protein (mg) | Total Activity (pmol/min)a | Specific Activity (pmol/min/mg)a | Purification Fold | Yield (%) |
|---|---|---|---|---|---|
| Crude extract | 812 | 1577.6 | 1.9 | 1 | 100 |
| (NH4)2SO4 pellet | 380 | 1520.0 | 4.0 | 2.1 | 96 |
| HIC column | 45.46 | 3957.4 | 87.1 | 44.8 | 250 |
| Heparin affinity | 12 | 2502.4 | 208.5 | 107.4 | 158 |
| Anion exchange | |||||
| SAS-1 | 0.082 | 844.3 | 10296.3 | 5307.4 | 53.5 |
| SAS-2 | 0.106 | 1087.7 | 10261.3 | 5289.3 | 68.9 |
| Size exclusion | |||||
| SAS-1 | 0.042 | 573.9 | 13664.3 | 7043.5 | 36.4 |
| SAS-2 | 0.054 | 739.4 | 13692.6 | 7058.1 | 46.9 |
a
1 pmol of substrate cleavage = 6.024 fluorescence units.