Table 3.

Effect of Different Salts on Hydrolytic Activity of SAS-1 and SAS-2

		Activity
Salt	Concentration	SAS-1	SAS-2
NaCl	10 mM	15 ± 1.6	11 ± 3.1
	100 mM	48 ± 7.1	41 ± 0.8
	500 mM	95 ± 2.7	100 ± 0.4
KCl	10 mM	13 ± 3.3	6 ± 5.0
	100 mM	42 ± 0.8	51 ± 3.4
	500 mM	108 ± 6.1	128 ± 10.7
CaCl2	10 mM	53 ± 3.1	65 ± 9.8
	100 mM	102 ± 4.3	118 ± 3.9
MgCl2	10 mM	50 ± 1.9	65 ± 2.1
	100 mM	90 ± 2.2	113 ± 3.7
ZnCl2	10 mM	16 ± 1.5	28 ± 2.8
	100 mM	20 ± 4.2	18 ± 5.6
ZnCl2 (with 500 mM NaCl)	10 mM	25 ± 6.1	32 ± 2.7
	100 mM	1 ± 0.5	1 ± 1.0

Partially purified enzymes assayed 1 h in indicated salt with the substrate Z-VAD-AFC. Activity is expressed as relative fluorescence units.