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. 2004 Apr;16(4):857–873. doi: 10.1105/tpc.017947

Table 7.

Effects of Various Protease Inhibitors on SAS-1 and SAS-2 Hydrolytic Activity

Percent Activity of Noninhibited
Inhibitor Preferred Protease Target SAS-1 SAS-2
No inhibitor - 100% ± 2.2% 100% ± 3.0%
Z-GF-NHO-Bza Subtilisin 1% ± 0.5% 1% ± 2.2%
Boc-APF-NHO-Bza Subtilisin 93% ± 4.2% 92% ± 3.8%
Aprotinin Ser 73% ± 8.1% 77% ± 2.8%
AEBSFb Ser 64% ± 5.4% 73% ± 4.2%
PMSFc Ser 60% ± 5.9% 70% ± 3.4%
Leupeptin Ser and Cys 88% ± 2.5% 81% ± 2.5%
Antipain Ser and Cys 100% ± 3.9% 100% ± 2.7%
E-64d Cys 79% ± 5.1% 81% ± 3.2%
PCMBe Thiol 89% ± 4.0% 100% ± 1.9%
Pepstatin A Aspartate 91% ± 2.5% 89% ± 4.1%

Purified SAS-1 and SAS-2 were preincubated with 200 μM inhibitor for 2 h, except PMSF (1 mM) and aprotinin (1 μg/mL), and then assayed for Z-VAD-AFC hydrolytic activity for 1 h.

a

Recognition sequence in single-letter amino acid code. NHO, nitrosyl; Bz, benzoyl.

b

4-(2-aminoethyl)-benzenesulfonyl-fluoride.

c

Phenylmethylsulfonyl fluoride.

d

Trans-epoxysuccinyl-l-leucylamido-(4-guanidino)-butane.

e

p-Chloromercuribenzoic acid.