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. Author manuscript; available in PMC: 2014 Aug 11.
Published in final edited form as: Mol Ther. 2007 Oct 23;16(1):187–193. doi: 10.1038/sj.mt.6300339

Figure 6. Cre-mediated excision of the internal ribosome entry site (IRES)–neo targeting cassette.

Figure 6

(a) Schematic representation of the COL1A2 locus depicting the untargeted, targeted, and Cre-excised targeted alleles. The locations of the stop codons (Stop), IRES-neo, loxP sites, and pA signal are shown. (b) Southern blot of NdeI/SalI-digested genomic DNA isolated from untransduced OIMSC12 cells (Untarg), a clone targeted at exon 2 of COL1A2 (Targ), and two targeted clones, after treatment with Cre recombinase (Cre). Expected fragment sizes obtained with probe p3 are 5.8 kilobase (kb) (untargeted alleles), 6.6 kb (targeted alleles), and 5.2 kb (Cre-excised targeted alleles). After Cre-mediated excision, the targeted allele retains stop codons in all three reading frames, a single loxP site, and a pA signal.