. Author manuscript; available in PMC: 2014 Aug 11.

Published in final edited form as: Mol Ther. 2007 Oct 23;16(1):187–193. doi: 10.1038/sj.mt.6300339

Table 2.

Results of adeno-associated virus-mediated gene targeting at COL1A2

Cell line	Exon Targeted^b	Multiplicity of infection	Plating efficiency (%)^c	G418^R colonies (%)^d	G418^R colonies analyzed^e	G418^R colonies targeted (#)^f	Unselected colonies targeted (%)^g
OIMSC12	4	2,000	70	0.16	20	65% (13)	0.10
OIMSC12	4	20,000	25	0.24	15	60% (9)	0.14
OIMSC12p^a	4	2,000	49	0.04	71	92%^*	0.04
OIMSC5	4	2,000	69	0.04	24	67% (16)	0.03
OIMSC1p^a	4	2,000	65	0.03	55	84%^*	0.03
OIMSC12	2	2,000	42	0.28	87	82% (71)	0.23

Polyclonal population.

Vectors AAV-COL2e4INloxpA (exon 4) or AAV-COL2e2INloxpA (exon 2).

Colonies obtained without selection per cell plated.

G418-resistant colonies/total number of colonies obtained.

For polyclonal populations, this represents the number of pooled colonies.

(Number of targeted colonies)/(total number of colonies analyzed) × 100.

Polyclonal results based on quantitative Southern blot analysis.

(% G418R colonies targeted) × (% G418R colonies).