Figure 6.

Control (A–D) and les (E–H) oligodendrocyte progenitor cells were fixed at 5 DIV and labeled with O1 (red) and DAPI (blue). At 3 DIV, the media was replaced with GDM or EBSS with or without CQ. Control and les progenitor cells differentiated into mature oligodendrocytes after incubation in GDM (A,E). Control oligodendrocytes were unaffected by the addition of CQ (B); however, les oligodendrocytes exhibited degraded membrane (thin arrows) and nuclear DNA fragmentation (block arrows) followed by incubation in GDM + CQ (F). Interestingly, les oligodendrocytes appeared to have more branching and cytoplasmic extensions after incubation in EBSS alone (G), while there was no obvious difference in the appearance of control oligodendrocytes (C). Both control and les oligodendrocytes exhibit signs of apoptosis after incubation in EBSS + CQ (D,H). Western blots performed on protein extracted from both samples confirmed that autophagy was upregulated in les oligodendrocytes (I), as there is a significant increase in LC3-II after incubation in EBSS + CQ (J). **p < 0.001, GDM versus EBSS + CQ treatments (Student's t test).